馃専寮€绡囩伒榄傛嫹闂細瀹為獙瀹よ悓鏂板繀鐪嬶紒

鍒氭帴瑙︾粏鑳炲疄楠岀殑浣狅紝鏄笉鏄鐫€鍩瑰吇鐨垮彂鎰侊紵鈥?strong>鈥嬫槑鏄庣粏鑳炲吇寰楃櫧鐧借儢鑳栵紝涓€鎻愮嚎绮掍綋灏辩炕杞︹€?/strong>鈥嬶紵鍒厡锛佷粖鍎垮挶灏卞敔鍞犳€庝箞浠庡煿鍏荤粏鑳為噷"鎺?鍑烘椿韫︿贡璺崇殑绾跨矑浣撱€傝浣忥紝杩欏彲涓嶆槸鎷嗘墜鏈烘崲鐢垫睜锛屽緱璁茬┒"娓╂煍涓€鍒€"锛?/p>

馃攳鍐昏瀺娉曪細缁欑粏鑳炶啘"鏉鹃"鐨勫啺鐏袱閲嶅ぉ

鈥?strong>鈥嬪煿鍏荤粏鑳炰负鍟ユ瘮鍔ㄧ墿缁勭粐闅炬悶锛熲€?/strong>鈥?鍥犱负瀹冧滑瑁圭潃榛忕硦绯婄殑缁嗚優澶栧熀璐紝鍍忕┛浜嗗眰闃插脊琛ｏ紒杩欐椂鍊欏氨寰楃敤鍐昏瀺娉曗€斺€斿師鐞嗙壒绠€鍗曪細璁╃粏鑳炶啘鍐昏鍑哄皬瀛旓紝鏂逛究鍚庣画鎿嶄綔銆?/p>

鈥?strong>鈥嬫搷浣滃彛璇€鈥?/strong>鈥嬶細

1. 鈥?strong>鈥嬪啺妗舵寫鎴樷€?/strong>鈥嬶細缁嗚優鎮恫鍐版荡10鍒嗛挓锛岃啘鏀剁缉鍙樿剢
2. 鈥?strong>鈥嬫€ュ喕缂撹瀺鈥?/strong>鈥嬶細-80鈩冮€熷喕20鍒嗛挓鈫?7鈩冩按娴村寲寮€锛屽惊鐜?娆?/li>
3. 鈥?strong>鈥嬭柉鐗囪啘璇炵敓鈥?/strong>鈥嬶細鏄惧井闀滀笅鐪嬶紝缁嗚優鑶滃竷婊″啺鏅惰绾?/li>

鈿狅笍鍒掗噸鐐癸細鈥?strong>鈥嬪喕铻嶈秴杩?娆?鑷潃琛屼负鈥?/strong>鈥嬶紒绾跨矑浣撹啘浼氳鍐版櫠鎴虫垚绛涘瓙锛屾椿鎬х洿鎺ユ帀鍒板湴鏉夸环銆?/p>

馃洜锔忔潨鎭╂柉鐮旂（鍣細瀹為獙瀹ょ殑"鎿€闈㈡潠"浣跨敤绉樼睄

杩欑幓鐠冪鍣ㄧ湅鐫€鍍忔崳钂滄澋锛岀敤璧锋潵璁茬┒鍙浜嗭紒鈥?strong>鈥嬫澗鏉电揣鏉靛垎涓嶆竻锛熸暀浣犱笁鎷涒€?/strong>鈥嬶細

鈥?strong>鈥嬭娉暀璁€?/strong>鈥嬶細

• 闅斿瀹為獙瀹ゅ皬鐜嬪繕璁板啺娴达紝缁撴灉铔嬬櫧閰舵毚璧帮紝绾跨矑浣撹鍟冨緱鍙墿娓?/li>
• 鐮旂（娑插繀椤绘饭娌℃澋澶?/3锛屽惁鍒欐皵娉′細鎶婄嚎绮掍綋鎸ょ牬锛堝埆闂垜鏄€庝箞鐭ラ亾鐨勶級

馃寑绂诲績涓夐噸濂忥細绛涘嚭绾跨矑浣撶殑绉樺瘑姝﹀櫒

绂诲績鏈哄彲涓嶆槸鎸変釜鎸夐挳灏卞畬浜嬶紒鈥?strong>鈥嬪弬鏁拌缃敊1绉掞紝涓夊ぉ瀹為獙鍏ㄧ櫧鎼炩€?/strong>鈥嬶細

1. 鈥?strong>鈥嬪垵绛涳紙1000g/10min锛夆€?/strong>鈥嬶細鐢╂帀缁嗚優鏍稿拰澶у潡澶?/li>
2. 鈥?strong>鈥嬬簿鎻愶紙12000g/15min锛夆€?/strong>鈥嬶細绾跨矑浣撶矖鍝乬et鈭?/li>
3. 鈥?strong>鈥嬬函鍖栵紙100000g/30min锛夆€?/strong>鈥嬶細鏍哥硸浣撶瓑灏忔潅纰庢嫓鎷?/li>

馃挕鐙鏁版嵁锛氶泤閰剁敓鐗╃殑涓ゆ瀵嗗害姊害娉曪紝绾害姣斾紶缁熸柟娉曢珮40%锛屼絾鏂版墜寤鸿鍏堜粠鏅€氱蹇冪粌鎵嬶紒

馃帹鏌撹壊涓変欢濂楋細缁欑嚎绮掍綋"绌胯。鏈?

鈥?strong>鈥嬭┕绾虫柉缁緽 vs MitoTracker锛屾€庝箞閫夛紵鈥?/strong>鈥?/p>

• 鈥?strong>鈥嬫墦宸ヤ汉棣栭€夆€?/strong>鈥嬶細瑭圭撼鏂豢B锛堜究瀹滃ぇ纰楋紝鏌撹壊蹇級
  閰嶆柟锛?%鍘熸恫1ml+49ml缂撳啿娑诧紝鐜扮敤鐜伴厤
• 鈥?strong>鈥嬪湡璞疄楠屽鈥?/strong>鈥嬶細MitoTracker Red锛堣兘鎶楀浐瀹氬鐞嗭紝閫傚悎鎷嶇編鍥撅級
  鏌撹壊绉樿瘈锛?7鈩冨鑲插埆瓒?0鍒嗛挓锛屽惁鍒欒崸鍏変細"瑜壊"

鈥?strong>鈥嬫樉寰暅涓嬬炕杞︾幇鍦衡€?/strong>鈥嬶細

• 鍏ㄦ槸缁胯壊鑽у厜锛熺嚎绮掍綋鑶滅數浣嶅穿浜嗭紒
• 鐪嬪埌绾㈣壊灏忓渾鐐癸紵鎭枩锛佹椿鎬у湪绾?/li>

馃毃閬垮潙鎸囧崡锛氳€佸徃鏈虹炕杩囩殑杞︿綘鍒炕

1. 鈥?strong>鈥嬫俯搴﹀け鎺ф儴妗堚€?/strong>鈥嬶細
   涓婂懆瀹為獙瀹ょ┖璋冨潖浜嗭紝灏忔潕鐨勭嚎绮掍綋娲绘€х洿鎺ヤ粠90%鎺夊埌20%銆傝浣忥細鈥?strong>鈥嬪叏绋?鈩冩搷浣滄槸搴曠嚎鈥?/strong>鈥嬶紒
2. 鈥?strong>鈥嬬蹇冪瑁呭弽濂囬椈鈥?/strong>鈥嬶細
   鏂版墜灏忓紶杩炵画12娆＄蹇冨け璐ワ紝鏈€鍚庡彂鐜扮瀛愯鍙?..锛堟唻绗戯紝浣犲彲鑳戒篃浼氾級
3. 鈥?strong>鈥嬪喕瀛樻恫閫夋嫨鐜勫鈥?/strong>鈥嬶細
   鐢ㄦ櫘閫氳敆绯栨恫鍐诲瓨锛屾椿鎬т繚鐣欎笉鍒?0%锛涙崲鎴愭捣钘荤硸缂撳啿娑诧紝鐩存帴椋欏埌95%

馃挰鑰佺鐮旂嫍鐨勭纰庡康

骞蹭簡鍏勾缁嗚優瀹為獙锛岃杩囧お澶?涓€鐪嬪氨浼氾紝涓€鍋氬氨搴?銆傗€?strong>鈥嬬嚎绮掍綋鍒嗙灏卞儚璋堟亱鐖扁€?/strong>鈥嬧€斺€斿お绮楁毚浼氫激鍒板畠锛屽お娓╂煍鍙堟悶涓嶅畾銆備釜浜哄缓璁細

• 璐村缁嗚優鍏堢敤鑳伴叾"鏉惧湡"锛屼絾娑堝寲鍒秴5鍒嗛挓锛堢粏鑳炰細鍝粰浣犵湅锛?/li>
• 瓒呭０鐮寸浠敤鏈€浣庡姛鐜囷紝1绉掑紑/2绉掑叧锛屽儚缁欑粏鑳炲仛鎸夋懇
• 涔颁笉璧烽珮绔瘯鍓傜洅锛熻瘯璇曡嚜鍒剁紦鍐叉恫锛?.25M钄楃硸+1mM EDTA锛屾垚鏈洿闄?0%

鏈€鍚庨€佸ぇ瀹朵竴鍙ワ細鈥?strong>鈥嬭澶囧彲浠ョ┓锛岃剳瀛愪笉鑳芥噿鈥?/strong>鈥嬶紒澶氱悽纾ㄥ師鐞嗭紝灏戞姳鎬ㄧ炕杞︼紝涓嬩竴涓疄楠屽澶х灏辨槸浣狅綖