首页 > 社会 > 正文内容

高效多糖纯化方法对比:胶柱层析与离子交换法的优劣解析

社会2025-05-28 08:26:27

馃殌銆愬紑绡囨毚鍑汇€?/h3>

浣犺杩囧疄楠屽閲岄偅浜涢粡绯婄硦鐨勫绯栨彁鍙栫墿鍚楋紵鍍忔瀬浜嗙啲杩囧ご鐨勮棔绮夛紝浣嗘兂鎶婂畠鍙樻垚鏅惰幑鍓旈€忕殑"鏄庢槦澶氱硸"锛屽彲姣斿啿钘曠矇闅句笂100鍊嶏紒浠婂ぉ鍜变滑灏辨潵鎺版壇鎺版壇瀹為獙瀹や袱澶ч晣瀹呮硶瀹濃€斺€斺€?strong>鈥嬭兌鏌卞眰鏋愨€?/strong>鈥嬪拰鈥?strong>鈥嬬瀛愪氦鎹㈡硶鈥?/strong>鈥嬶紝鐪嬬湅瀹冧滑鍒板簳璋佹墠鏄绯栫函鍖栫殑"鎵涙妸瀛?锛?/p>


馃挕 鈥?strong>鈥嬩竴銆佷负浠€涔堢函鍖栧绯栬繖涔堣绌讹紵鈥?/strong>鈥?/h4>

涓句釜鏍楀瓙馃尠锛氭煇鑽巶鐢ㄦ湭绾寲鐨勭伒鑺濆绯栧仛鎶楃檶瀹為獙锛岀粨鏋滄椿鎬ф垚鍒嗚鏉傝川铔嬬櫧璐?鎷栧悗鑵?锛岃嵂鏁堢洿鎺ヨ叞鏂╋紒鈥?strong>鈥嬬函搴︽瘡鎻愬崌1%锛屽姛鏁堝彲鑳界炕鍊嶁€?/strong>鈥嬩綘淇′笉锛熸墍浠ヨ锛岄€夊绾寲鏂规硶灏卞儚閫夊璞♀€斺€斿悎閫傛渶閲嶈锛?/p>


鈿栵笍 鈥?strong>鈥嬩簩銆佷袱澶ч棬娲剧‖鏍窹K鈥?/strong>鈥?/h3>

鍏堢粰鏂版墜鍒掗噸鐐癸紒杩欎咯鏂规硶鍚勬湁缁濇椿锛屽挶浠敤鏈€鐩寸櫧鐨勪汉璇濆姣斾笅锛?/p>

鈥?strong>鈥嬪姣旈」鈥?/strong>鈥?/th>鈥?strong>鈥嬭兌鏌卞眰鏋愨€?/strong>鈥?/th>鈥?strong>鈥嬬瀛愪氦鎹㈡硶鈥?/strong>鈥?/th>
鐪嬪鏈鎸夊绯?浣撳瀷"绛涘垎锛堝垎瀛愰噺鍒嗙骇锛?/td>鎸夊绯?鎬ф牸"绛涢€夛紙鐢佃嵎鐗规€э級
閫傜敤鍦烘櫙鑴辩洂銆佸垎瀛愰噺鍒嗙骇銆佸幓灏忓垎瀛愭潅璐?/td>鍒嗙閰告€?涓€у绯?/td>
瑁呭瑕佹眰钁¤仛绯栧嚌鑳讹紙Sephadex绯诲垪鏈€甯歌锛?/td>DEAE/CM绾ょ淮绱犳煴锛堥槾闃崇瀛愶級
鎿嶄綔闅惧害鏂版墜鍙嬪ソ锛屾祦绋嬫爣鍑嗗寲闇€绮惧噯鎺у埗pH鍜岀瀛愬己搴?/td>
鐑ч挶鎸囨暟鑰楁潗渚垮疁浣嗚€楁椂闀?/td>鏌卞瓙璐典絾鏁堢巼楂?/td>

锛堟暟鎹患鍚堣嚜缃戦〉1/3/4/8锛?/p>


馃И 鈥?strong>鈥嬩笁銆佽兌鏌卞眰鏋愶細澶氱硸鐣岀殑浣撻噸绉も€?/strong>鈥?/h3>

鈥?strong>鈥嬪師鐞嗘彮绉樷€?/strong>鈥嬶細灏卞儚鐢ㄧ瓫瀛愮瓫绫筹紝澶ч绮掑绯栬鎸″湪鍑濊兌瀛斿鍏堣窇璺紝灏忎釜瀛愬绯栭捇杩峰浼煎湴鎱㈡參鐖€?br/> 鈥?strong>鈥嬪疄鎴樹笁浠跺鈥?/strong>鈥嬶細

  1. 鈥?strong>鈥嬮€夊嚌鑳垛€?/strong>鈥嬶細Sephadex G绯诲垪鏄爣閰嶏紝G鍚庨潰鐨勬暟瀛楄秺澶э紝鑳界瓫鐨勫垎瀛愰噺鑼冨洿瓒婂箍
  2. 鈥?strong>鈥嬭鏌辩巹瀛︹€?/strong>鈥嬶細鏌卞瓙瑕佽寰楀儚鍗冨眰铔嬬硶鑸潎鍖€锛屾檭鍑烘皵娉″氨缈昏溅锛?/li>
  3. 鈥?strong>鈥嬫礂鑴辩璇€鈥?/strong>鈥嬶細缂撳啿娑查噷鍔犵偣鐩愰槻鍚搁檮锛屾祦閫熸帶鍒跺湪姣忓垎閽?ml宸﹀彸

鈿狅笍鈥?strong>鈥嬭娉暀璁€?/strong>鈥嬶細鍘诲勾闅斿瀹為獙瀹ょ敤G-50鏌卞垎绂婚鑿囧绯栵紝缁撴灉涓婃牱閲忚秴5%锛屽嚭鏉ョ殑宄版瘮蹇冪數鍥捐繕涔憋紒


鈿?鈥?strong>鈥嬪洓銆佺瀛愪氦鎹㈡硶锛氱數鑽锋崟鎵嬬殑鑹烘湳鈥?/strong>鈥?/h3>

鈥?strong>鈥嬫牳蹇冮€昏緫鈥?/strong>鈥嬶細甯﹁礋鐢电殑澶氱硸锛堟瘮濡傛捣钘诲绯栵級浼氭姝绘姳浣廌EAE绾ょ淮绱犳煴锛屾礂鑴辨椂鐢ㄤ笉鍚岀洂娴撳害"鎺板紑"瀹冧滑鐨勫皬鎵嬨€?br/> 鈥?strong>鈥嬫柊鎵嬪繀鐭ヤ笁瑕佺礌鈥?/strong>鈥嬶細

  1. 鈥?strong>鈥媝H鍊煎畾涔惧潳鈥?/strong>鈥嬶細閰告€у绯栬鍦╬H2-4鎵嶈兘涔栦箹涓婃煴
  2. 鈥?strong>鈥嬬洂姊害娲楄劚鈥?/strong>鈥嬶細浠?.1M鍒?.5M NaCl姝ユ涓鸿惀
  3. 鈥?strong>鈥嬪啀鐢熻褰诲簳鈥?/strong>鈥嬶細鐢?M NaOH娲楁煴瀛愭瘮娲楃杩橀噸瑕侊紒

鉁呪€?strong>鈥嬬湡瀹炴渚嬧€?/strong>鈥嬶細鐢–M绾ょ淮绱犳煴绾寲榛勮姫澶氱硸锛岄€氳繃0.3M NaCl娲楄劚寰楀埌鐨勪富鎴愬垎绾害鐩存帴椋欏埌95%锛?/p>


馃挵 鈥?strong>鈥嬩簲銆佺┓鏈夌┓鐜╂硶 瀵屾湁瀵岄棬閬撯€?/strong>鈥?/h3>

鈥?strong>鈥嬮绠楀悆绱ф€庝箞鍔炩€?/strong>鈥嬶細

  1. 鍏堢敤閱囨矇娉曠矖鎻愶紙涔欓唶娴撳害姊害娉曪級
  2. 鎺ヨ兌鏌卞眰鏋愬幓灏忓垎瀛?/li>
  3. 鏈€鍚庤繃绂诲瓙浜ゆ崲鏌辩簿鍒?/li>

鈥?strong>鈥嬪湡璞疄楠屽濂楅鈥?/strong>鈥嬶細
瓒呮护闄ゆ潅鈫掔瀛愪氦鎹㈡煴鍒嗙骇鈫掑嚌鑳舵煴绮句慨鈫掑喕骞蹭繚瀛?br/> 锛堣繖濂楃粍鍚堟嫵涓嬫潵锛岀函搴﹁兘鍒?9%+锛屼絾鐑ч挶鎸囨暟浜旈鏄燂級


馃敭 鈥?strong>鈥嬬嫭瀹惰瑙b€?/strong>鈥?/h3>

鏈€杩戝彂鐜颁釜鏈夎叮鐜拌薄锛氣€?strong>鈥嬮摐绂诲瓙娌夋穩娉曗€?/strong>鈥嬪湪鍘婚櫎铔嬬櫧璐ㄦ椂锛屾瘮浼犵粺Sevag娉曟晥鐜囬珮30%锛佽櫧鐒舵搷浣滈夯鐑︾偣锛屼絾瀵规煇浜涚壒娈婂绯栧彲鑳芥槸鏉℃柊璺瓙銆傝瘽璇村洖鏉ワ紝杩欎咯鏂规硶鍏跺疄鍙互鎼?鑱斿Щ"鈥斺€斿厛鐢ㄧ瀛愪氦鎹㈡硶鎸夌數鑽峰垎绫伙紝鍐嶇敤鑳舵煴灞傛瀽绛涘垎瀛愰噺锛屾晥鏋滅洿鎺?+1>2锛?/p>


馃搶 鈥?strong>鈥嬫渶鍚庡垝閲嶇偣鈥?/strong>鈥?/h4>

璁颁綇杩欎笁鏉♀€?strong>鈥嬩繚鍛藉彛璇€鈥?/strong>鈥嬶細

  1. 鑳舵煴灞傛瀽鍓嶅姟蹇呰劚铔嬬櫧锛屽惁鍒欐煴瀛愬彉"鑵婅偁"
  2. 绂诲瓙浜ゆ崲娲楄劚姊害瑕佸钩缂擄紝蹇冩€ュ悆涓嶄簡鐑眴鑵?/li>
  3. 涓ょ鏂规硶娣锋惌鐢紝鐪侀挶鐪佸姏鍑哄杩?/li>

涓嬫鍐嶈鍒伴偅鍧?榛忕硦鎬?锛屾槸涓嶆槸瑙夊緱瀹冪湁娓呯洰绉€澶氫簡锛熸悶绉戠爺灏卞儚鎵撴父鎴忥紝鎺屾彙鎶€鑳藉寘鎵嶈兘閫氬叧BOSS锛?/p>

搜索